Glycoprotein receptors for a heat-stable enterotoxin (STh) produced by enterotoxigenic Escherichia coli
نویسندگان
چکیده
منابع مشابه
Heat stable enterotoxin produced by Escherichia coli in acute diarrhoea.
We screened 569 children with acute onset diarrhoea from five Italian towns for heat stable enterotoxin produced by Escherichia coli. We compared an enzyme linked immunoabsorbent assay (ELISA) with the standard suckling mouse assay for detection of the enterotoxin. A total of 31 (5.4%) children were found who had strains of E coli that produced heat stable enterotoxin: 26 strains were positive ...
متن کاملStructural Characterisation of the E. coli Heat Stable Enterotoxin STh
E. coli heat stable enterotoxin STa is an agonist of the membrane guanylate cyclase C whose endogenous ligands are the peptide hormones guanylin and uroguanylin. Whereas these peptides contain only two disulfide bonds, STa is stabilized by one additional disulfide bridge. We chemically synthesized the enterotoxin STh that originates from the E. coli strain found in humans, and we determined its...
متن کاملProperties of synthetically produced Escherichia coli heat-stable enterotoxin.
The properties of a synthetically produced peptide composed of the same primary structure of 18 amino acids described for human Escherichia coli heat-stable enterotoxin were compared with those of purified heat-stable toxin obtained by bacterial growth. The dosage required to evoke fluid secretion in the suckling mouse and rat ligated ileal loop assays was the same for both toxins. The antigeni...
متن کاملValue of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.
The method of passive immune hemolysis of Evans and Evans (Infect. Immun. 16:604-609, 1977) for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli was modified. A total of 373 strains of E. coli were tested by this method using materials obtained by treating the cells with polymyxin B and rabbit antiserum against cholera enterotoxin, purified by affinity gel colum...
متن کاملComparison of three assay systems for detection of enterotoxigenic Escherichia coli heat-stable enterotoxin.
In this study, a commercial DNA-DNA hybridization kit for the detection of Escherichia coli heat-stable enterotoxin is compared with a competitive enzyme-linked immunosorbent assay (ELISA) and the suckling mouse bioassay. Taking the suckling mouse assay as the "gold standard," the gene probe was the more specific and the ELISA was the more sensitive of the assays. The ELISA and the suckling mou...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1992
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.60.10.4213-4220.1992